| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil
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| Autores: |
Albuquerque,Pedro Paulo Feitosa de
Santos,André de Souza
Souza Neto,Orestes Luiz de
Kim,Pomy de Cássia Peixoto
Cavalcanti,Erika Fernanda Torres Samico Fernandes
Oliveira,Júnior Mário Baltazar de
Mota,Rinaldo Aparecido
Pinheiro Júnior,José Wilton
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| Data: |
2017-03-01
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| Ano: |
2017
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| Palavras-chave: |
Molecular biology
Diagnosis
Paratuberculosis
Milk
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| Resumo: |
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was found in bovine milk samples from the State of Pernambuco. To the best of our knowledge, this is the first report of MAP DNA found in bovine milk in Northeast Brazil. We also demonstrated the qPCR technique is more sensitive than conventional PCR with respect to detection of MAP in milk samples.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2016.10.010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.48 n.1 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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